rabbit monoclonal anti mafa d2z6n (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rabbit monoclonal anti mafa d2z6n
Rabbit Monoclonal Anti Mafa D2z6n, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mafa/MAFA+Rabbit+mAb/pmc13049674-2-0-5
Average 95 stars, based on 37 article reviews
Rabbit Monoclonal Anti Mafa D2z6n, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mafa/MAFA+Rabbit+mAb/pmc13049674-2-0-5
Average 95 stars, based on 37 article reviews
rabbit monoclonal anti mafa d2z6n - by Bioz Stars,
2026-09
95/100 stars
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Blocking Assay:Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3. Article Snippet: 5X protein loading buffer (Beyotime Biotechnology, P0286) was added to the residual supernatants and boiled for 20 min. About 30mg of total protein was subjected to sodium dodecyl-sulfate polyacrylamide gel (SDS-PAGE) electrophoresis, and transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% BSA for 1.5h, membranes were incubated overnight at 4 C with the following primary antibodies at a dilution of 1:1000: b-Actin (Cell Signaling Technology, 4970), STAT3 (Cell Signaling Technology, 4904), p-STAT3Tyr705 (Cell Signaling Technology, 9131), IL22RA1 (Invitrogen, PA5-19987), c-Jun (Cell Signaling Technology, 9165), p-JunSer73 (Cell Signaling Technology, 9164), CYB5R3 (Proteintech, 10894-1- AP), OXPHOS (Abcam, ab110413), Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3. Article Snippet: .. Antigen retrieval was carried out by boiling in Tris-EDTA buffer or citrate buffer for microwaving 10 min. After cooling to room temperature, sections were covered in blocking buffer (5% donkey serum in PBS) for 1 h. Primary antibodies were applied at a dilution of 1:400 for INSULIN (Cell Signaling Technology, 8138), 1:400 for GLUCAGON (Cell Signaling Technology, 2760), 1:50 for IL22RA1 (R&D SYSTEM, 496514), 1:100 for CYB5R3 (Proteintech, 10894-1-AP), 1:100 for Incubation:Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3. Article Snippet: 5X protein loading buffer (Beyotime Biotechnology, P0286) was added to the residual supernatants and boiled for 20 min. About 30mg of total protein was subjected to sodium dodecyl-sulfate polyacrylamide gel (SDS-PAGE) electrophoresis, and transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% BSA for 1.5h, membranes were incubated overnight at 4 C with the following primary antibodies at a dilution of 1:1000: b-Actin (Cell Signaling Technology, 4970), STAT3 (Cell Signaling Technology, 4904), p-STAT3Tyr705 (Cell Signaling Technology, 9131), IL22RA1 (Invitrogen, PA5-19987), c-Jun (Cell Signaling Technology, 9165), p-JunSer73 (Cell Signaling Technology, 9164), CYB5R3 (Proteintech, 10894-1- AP), OXPHOS (Abcam, ab110413), Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3. Article Snippet: .. Antigen retrieval was carried out by boiling in Tris-EDTA buffer or citrate buffer for microwaving 10 min. After cooling to room temperature, sections were covered in blocking buffer (5% donkey serum in PBS) for 1 h. Primary antibodies were applied at a dilution of 1:400 for INSULIN (Cell Signaling Technology, 8138), 1:400 for GLUCAGON (Cell Signaling Technology, 2760), 1:50 for IL22RA1 (R&D SYSTEM, 496514), 1:100 for CYB5R3 (Proteintech, 10894-1-AP), 1:100 for Western Blot:Article Title: Obesity-induced upregulation of miR-483-5p impairs the function and identity of pancreatic β-cells. Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: 32171116, 32371168, 82370803, 82170810; Research Project of Taizhou Clinical Medical College of Nanjing Medical University, Grant/Award Number: TZKY20230310 Abstract Aim: To assess the expression and function of miR-483-5p in diabetic β cells.. Methods: The expression of miR-483-5p was evaluated in the pancreatic islets of obesity mouse models by quantitative reverse transcription polymerase chain reaction.. Dual-luciferase activity, and western blotting assays, were utilized for miR483-5p target gene verification. Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway Article Snippet: .. The antibodies used in WB were: INSULIN (1:1000, ab181547, Abcam), PPARδ (1:1000, 74076 S, CST), UCP2 (1:1000, 89326 S, CST), PDX1 (1:1000, ab219207, Abcam), MAFA (1:1000, 7937 S, CST), IRS1 (1:1000, 2390 S, CST), GCK (1:1000, 19666-1-AP, Proteintech), β-ACTIN (1:1000, PM053, MBL) and β-TUBULIN (1:1000, 2128 S, CST). .. For quantification, target protein band intensities were normalised against β-TUBULIN and analysed by densitometry using ImageJ software. Article Title: Huanglian-Renshen-Decoction Maintains Islet β-Cell Identity in T2DM Mice through Regulating GLP-1 and GLP-1R in Both Islet and Intestine. Article Snippet: Type 2 diabetes mellitus (T2DM) is a metabolic disease that severely threatens human health and general well-being.. According to a survey by the International Diabetes Federation, 400 million patients are diagnosed with T2DM yearly, of whom 9 million are critically ill. T2DM results from insulin resistance and β-cell dysfunction, gradually leading to uncontrollable hyperglycemia.. According to the latest research, islet β-cell identity loss is one of the mechanisms of T2DM, contributing to insufficient insulin secretion; islet β-cells transdifferentiate into other cells without insulin secretion function. |
